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Primary Cultures Are Immortalized True of False Questions

Primary Cultures Are Immortalized True of False Questions

Question Description

True of False

1.Primary cultures are immortalized

2.Primary cultures have a limit of 10-12 passages

3.Primary cultures are better source for cell lines

4.Presence of Ca and Mg ions in the medium is required for cell attachment to the surface

5.Feeder layer are dead cells

6.Mycoplasma contamination of cell cultures is the most difficult one to deal with

7.The optimal temperature to grow most of animal cells is 32-33 C degree

8.The optimal air content for cultured cells is 5% CO2

9.Fetal bovine serum concentration in the culture medium is usually 5%

10. Contact inhibition is inhibition of growth when cells reach 90-100 confluency

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